Apoptotic effect of Bulbine Natalensis and Chlorophytum Comosum in myelogenous Leukemia K562 cell line / Efeito apoptótico da natalensia bulbina e do clorofito comoso na linhagem celular mielogênea da leucemia K562

Abstract Bulbine natalensis and Chorophytum comosum are potential medicinal source for the treatment of cancers. Chronic myeloid leukaemia is a hematopoietic stem cells disorder treated by tyrosine kinase inhibitors but often cause recurrence of the leukaemia after cessation of therapy, hence require alternative treatment. This study determines the anti-cancer effect of leaf, root and bulb methanolic and aqueous extracts of B. natalensis and C. comosum in chronic human myelogenous leukaemia (K562) cell line by MTT, Hoechst bis-benzimide nuclear and annexin V stain assays. The root methanolic extract of B. natalensis and C. comosum showed a high cytotoxicity of 8.6% and 16.7% respectively on the K562 cell line at 1,000 μg/ml concentration. Morphological loss of cell membrane integrity causing degradation of the cell and fragmentation were observed in the root methanolic extract of both plants. A high apoptosis (p < 0.0001) was induced in the K562 cells by both leaf and root extracts of the C. comosum compared to the B. natalensis. This study shows both plants possess apoptotic effect against in vitro myelogenous leukaemia which contributes to the overall anti-cancer properties of B. natalensis and C. comosum to justify future therapeutic applications against chronic myelogenous leukaemia blood cancer.

Resumo Bulbine natalensis Baker e Chorophytum comosum (Thunb.) Jacques são potenciais fontes medicinais para o tratamento de cânceres. A Leucemia Mieloide Crônica (LMC) é um distúrbio das células-tronco hematopoiéticas que é tratado com inibidores da tirosina quinase, mas frequentemente, causa recorrência da leucemia após a interrupção da terapia, portanto, requer um tratamento alternativo. Este estudo determinou o efeito anticancerígeno de extratos metanólicos e aquosos de folha, raiz e bulbo de B. natalensis e C. comosum na linhagem celular de leucemia mieloide humana crônica (K562) por ensaios de MTT, Hoechst bis-benzimida nuclear e anexina V. O extrato metanólico da raiz de B. natalensis e C. comosum apresentou alta citotoxidade de 8,6% e 16,7% respectivamente, na linhagem celular K562 com a concentração de 1,000 μg / ml. Perda morfológica da integridade da membrana celular causando degradação dos núcleos, citoplasma e encolhimento celular foi observada no extrato metanólico da raiz de ambas as plantas. Uma alta apoptose (p <0,0001) foi induzida nas células K562 por extratos de folhas e raízes de C. comosum em comparação com B. natalensis. Este estudo mostrou que ambas as plantas possuem efeito apoptótico contra leucemia mieloide in vitro que contribui para as propriedades anticâncer gerais de B. natalensis e C. comosum para justificar futuras aplicações terapêuticas contra câncer de sangue de LMC.

Apoptotic effect of Bulbine Natalensis and Chlorophytum Comosum in myelogenous Leukemia K562 cell line

Abstract Bulbine natalensis and Chorophytum comosum are potential medicinal source for the treatment of cancers. Chronic myeloid leukaemia is a hematopoietic stem cells disorder treated by tyrosine kinase inhibitors but often cause recurrence of the leukaemia after cessation of therapy, hence require alternative treatment. This study determines the anti-cancer effect of leaf, root and bulb methanolic and aqueous extracts of B. natalensis and C. comosum in chronic human myelogenous leukaemia (K562) cell line by MTT, Hoechst bis-benzimide nuclear and annexin V stain assays. The root methanolic extract of B. natalensis and C. comosum showed a high cytotoxicity of 8.6% and 16.7% respectively on the K562 cell line at 1,000 g/ml concentration. Morphological loss of cell membrane integrity causing degradation of the cell and fragmentation were observed in the root methanolic extract of both plants. A high apoptosis (p 0.0001) was induced in the K562 cells by both leaf and root extracts of the C. comosum compared to the B. natalensis. This study shows both plants possess apoptotic effect against in vitro myelogenous leukaemia which contributes to the overall anti-cancer properties of B. natalensis and C. comosum to justify future therapeutic applications against chronic myelogenous leukaemia blood cancer.

Resumo Bulbine natalensis Baker e Chorophytum comosum (Thunb.) Jacques são potenciais fontes medicinais para o tratamento de cânceres. A Leucemia Mieloide Crônica (LMC) é um distúrbio das células-tronco hematopoiéticas que é tratado com inibidores da tirosina quinase, mas frequentemente, causa recorrência da leucemia após a interrupção da terapia, portanto, requer um tratamento alternativo. Este estudo determinou o efeito anticancerígeno de extratos metanólicos e aquosos de folha, raiz e bulbo de B. natalensis e C. comosum na linhagem celular de leucemia mieloide humana crônica (K562) por ensaios de MTT, Hoechst bis-benzimida nuclear e anexina V. O extrato metanólico da raiz de B. natalensis e C. comosum apresentou alta citotoxidade de 8,6% e 16,7% respectivamente, na linhagem celular K562 com a concentração de 1,000 g / ml. Perda morfológica da integridade da membrana celular causando degradação dos núcleos, citoplasma e encolhimento celular foi observada no extrato metanólico da raiz de ambas as plantas. Uma alta apoptose (p 0,0001) foi induzida nas células K562 por extratos de folhas e raízes de C. comosum em comparação com B. natalensis. Este estudo mostrou que ambas as plantas possuem efeito apoptótico contra leucemia mieloide in vitro que contribui para as propriedades anticâncer gerais de B. natalensis e C. comosum para justificar futuras aplicações terapêuticas contra câncer de sangue de LMC.

Pot study using Chlorophytum comosum plants to biomonitor PAH levels in domestic kitchens.

In indoor environments, cooking is a major contributor to indoor air pollution releasing potentially harmful toxic compounds such as polycyclic aromatic hydrocarbons. In our study, Chlorophytum comosum 'Variegata' plants were applied to monitor PAH emission rates and patterns in previously selected rural Hungarian kitchens. Concentration and profile of accumulated PAHs could be well explained by cooking methods and materials used in each kitchen. Accumulation of 6-ring PAHs was characteristic in the only kitchen which frequently used deep frying. It also should be emphasized that applicability of C. comosum as indoor biomonitor was assessed. The plant has proven a good monitor organism as it accumulated both LMW and HMW PAHs.

New Insight into Short Time Exogenous Formaldehyde Application Mediated Changes in Chlorophytum comosum L. (Spider Plant) Cellular Metabolism.

Chlorophytum comosum L. plants are known to effectively absorb air pollutants, including formaldehyde (HCHO). Since the metabolic and defense responses of C. comosum to HCHO are poorly understood, in the present study, biochemical changes in C. comosum leaves induced by 48 h exposure to exogenous HCHO, applied as 20 mg m-3, were analyzed. The observed changes showed that HCHO treatment caused no visible harmful effects on C. comosum leaves and seemed to be effectively metabolized by this plant. HCHO application caused no changes in total chlorophyll (Chl) and Chl a content, increased Chl a/b ratio, and decreased Chl b and carotenoid content. HCHO treatment affected sugar metabolism, towards the utilization of sucrose and synthesis or accumulation of glucose, and decreased activities of aspartate and alanine aminotransferases, suggesting that these enzymes do not play any pivotal role in amino acid transformations during HCHO assimilation. The total phenolic content in leaf tissues did not change in comparison to the untreated plants. The obtained results suggest that HCHO affects nitrogen and carbohydrate metabolism, effectively influencing photosynthesis, shortly after plant exposure to this volatile compound. It may be suggested that the observed changes are related to early HCHO stress symptoms or an early step of the adaptation of cells to HCHO treatment. The presented results confirm for the first time the direct influence of short time HCHO exposure on the studied parameters in the C. comosum plant leaf tissues.

Effect of farmyard manure, elemental sulphur and EDTA on growth and phytoextraction of cadmium by spider plants (Chlorophytum comosum L.) under Cd stress.

Cadmium (Cd) contamination is considered as a widespread concern at global scale which is serious threats to human health. Phytoremediation is an eco-friendly approach which can remove or immobilize Cd from the soil. Different organic and inorganic amendments can potentially enhance Cd phytoremediation efficiency but the comparison of farmyard manure (FM), elemental sulphur (S) and ethylenediaminetetraacetic acid (EDTA) for Cd phytoremediation through spider plants (Chlorophytum comosum L.) remained unanswered. The present study evaluated the efficiency of S (0.1 and 0.2%), EDTA (0.1 and 0.2%, represented as EDTA-0.1 and EDTA-0.2) and FM (0.5 and 1%, represented as FM-0.5 and FM-1) for remediation of Cd contaminated soils (50 and 100 mg kg-1, represented as Cd-50 and Cd-100) through spider plants. Results depicted that the highest shoots and roots dry biomass was found in FM treated plants followed by S, EDTA and control except in EDTA-0.2 treatment in which the lowest values of these parameters were observed. Application of FM-1 significantly increased the shoot dry weight (120%), root dry weight (99%), as well as photosynthetic attributes in Cd-50 as compared to control. Application of EDTA-0.2 increased the bioavailable fraction of Cd than control and the maximum increase was observed in Cd-100. The highest Cd concentrations in shoot and roots were found in EDTA treated plants followed by S, control and FM irrespective of Cd and amendment levels. Maximum Cd in roots (109%) and shoots (156%) was recorded in plants grown in Cd-100 with EDTA-0.2 than control. The maximum bioaccumulation factor, translocation index, harvest index and root to shoot translocation were observed with EDTA than control and other treatments. EDTA along with spider plants may enhance the uptake of Cd but lower biomass production in the highest dose of EDTA may questioned the efficiency of EDTA.

Phytochemical Characterization, Antioxidant Activity, and Cytotoxicity of Methanolic Leaf Extract of Chlorophytum Comosum (Green Type) (Thunb.) Jacq.

Chlorophytum genus has been extensively studied due to its diverse biological activities. We evaluated the methanolic extract of leaves of Chlorophytum comosum (Green type) (Thunb.) Jacques, the species that is less studied compared to C. borivilianum. The aim was to identify phytoconstituents of the methanolic extract of leaves of C. comosum and biological properties of its different fractions. Water fraction was analyzed with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Nineteen compounds belonging to different chemical classes were identified in the methanolic extract of leaves of C. comosum (Green type) (Thunb.) Jacques. In addition to several fatty acids, isoprenoid and steroid compounds were found among the most abundant constituents. One of the identified compounds, 4'-methylphenyl-1C-sulfonyl-ß-d-galactoside, was not detected earlier in Chlorophytum extracts. The water fraction was toxic to HeLa cells but not to Vero cells. Our data demonstrate that methanolic extract of leaves of C. comosum can be a valuable source of bioactive constituents. The water fraction of the extract exhibited promising antitumor potential based on a high ratio of HeLa vs. Vero cytotoxicity.

[Effects of cadmium stress on growth and cadmium enrichment of Chlorophytum comosum and Chlorophytum comosum var. variegatum.] / é•‰èƒè¿«å¯¹åŠå °åŠé“¶è¾¹åŠå °ç”Ÿé•¿åŠé•‰å¯Œé›†ç‰¹æ€§çš„å½±å“.

We examined the growth and physiological characteristics of Chlorophytum comosum and Chlorophytum comosum var. variegatum by hydroponics at different Cd2+ concentrations (0, 20, 80, 200 µmol·L-1). The results showed that 20 µmol·L-1 Cd2+ did not affect those two varieties, with no changes of single leaf area, total leaf area, chlorophyll (Chl) a content, Chl (a+b) content, carotenoid content, Chla/Chlb value, intercellular carbon dioxide concentration (Ci) and transpiration rate (Tr) compared with the control (CK). Under the 80 µmol·L-1 Cd2+ stress, the initial fluorescence (Fo) and non-photochemical quenching coefficient (NPQ) were the highest for both varieties. As for Cd2+ at 200 µmol·L-1, the biomass, chlorophyll content, maximum net photosynthetic rate (Pn), stomatal conductance (gs), maximum photochemical quantum yield (Fv/Fm), actual photochemical quantum yield Y(II), the transfer factor (TF) of both varieties and all parts biomass decreased to the lowest, while peroxidase (POD), ascorbic acid peroxidase (APX) and catalase (CAT) activities of two varieties and MDA content of C. comosum var. variegatum increased in different degrees. With the increases of Cd2+ stress, the Cd content in organs of two varieties showed an increasing trend, with higher Cd accumulation in roots. C. comosum had higher Cd content and bioconcentration factor (BCF) in all organs than C. comosum var. variegatum. It suggested that two varieties had the ability to resist Cd stress. The tolerance of C. comosum was stronger, and thus could be considered as a green plant to remediate Cd contaminated water or soil.

Green synthesis of iron-based nanoparticles using Chlorophytum comosum leaf extract: methyl orange dye degradation and antimicrobial properties.

Nowadays, green synthesis methods have gained growing attention in nanotechnology owning to their versatile features including high efficiency, cost-effectiveness, and eco-friendliness. Here, the aqueous extract of Chlorophytum comosum leaf was applied for the preparation of iron nanoparticles (INPs) to obtain spherical and amorphous INPs with a particle size below 100 nm as confirmed by TEM. The synthesized INPs managed to eliminate methyl orange (MO) from the aqueous solution. The concentration of MO can be easily checked via ultraviolet-visible (UV-Vis) spectroscopy throughout the usage of INPs at the presence of H2O2. The synthesized INPs exhibited MO degradation efficiency of 77% after 6 h. Furthermore, the synthesized INPs exhibited antibacterial activity against both Gram-negative and Gram-positive bacteria. The prepared INPs have an impressive effect on Staphylococcus aureus at concentrations below 6 µg/ml. Overall, the synthesized INPs could considerably contribute to our combat against organic dyes and bacteria.

Chemical Profiling of Chlorophytum comosum (Thunb.) Jaques by GC-MS/LC-ESIMS and its Antiproliferative Effects on Human Carcinoma Cell Lines.

BACKGROUND: Chlorophytum comosum, popularly known as Spider Ivy, is used as a medicinal plant in traditional Chinese medicine, however, its detailed chemical composition and biological activity are yet unexplored. OBJECTIVE: To carry out the phytochemical investigation on different parts of Chlorophytum comosum using GCMS/ LC-ESI-MS and evaluation of its antioxidant, hemolytic and antiproliferative potential on breast cancer (MCF-7), lung cancer (A549, H1299) and normal lung (L-132) cell lines. METHODS: Chemical constituents from aqueous roots and leaves extracts were identified using LC-ESI-MS/GCMS. The identified compounds were annotated based on the match of mass spectra with the literature using NIST 14 and METLIN databases. Antioxidant activity was studied using DPPH, FRAP and TPC assays. The antiproliferative effects of ethanolic roots and leaves extracts of Chlorophytum comosum were measured by MTT assay on breast cancer (MCF-7), lung cancer (A549 & H1299) and normal lung (L-132) cell lines. The toxicity studies of the extracts were carried out using Hemolysis assay. RESULTS: GC-MS analysis identified 34 metabolites in roots and 17 from leaves, while 17 compounds from roots and 7 from leaves were detected by LC-ESI-MS. Significant antiproliferative effects were observed on the A549 and MCF-7 cancer cell lines with IC50 values ranging from 56.86 µg/ml to 68.68 µg/ml while no marked response was observed against normal cell line L-132. CONCLUSION: Our study represents the first report on the detailed chemical composition and antiproliferative potential of Chlorophytum comosum against lung and breast cancer cell lines.

Characterization of the complete chloroplast genome of Chlorophytum comosum (Liliaceae).

Chlorophytum comosum is a perennial ornamental plant in the family Liliaceae, it is also a valuable medicinal plant. To enrich the genetic resources of C. comosum, its chloroplast genome was determined by Illumina sequencing data. The chloroplast genome is a typical quadripartite structure with a size of 153,983 bp, of which the LSC region is 83,471 bp, the SSC region is 18,010 bp, and the pair of IR regions is 26,251 bp. The overall GC content is 37%. It contains 131 genes, including 85 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. Phylogenetic analyses showed that C. comosum is closely related to Chlorophytum rhizopendulum. However, it can be distinguished from other plants. This study enriches the sequence resources of C. comosum and provides important data for the development of molecular identification markers.

Indoor formaldehyde removal by three species of Chlorphytum Comosum under the long-term dynamic fumigation system.

Gaseous formaldehyde removal efficiency and physiological characteristics of leaves were investigated through a dynamic fumigation system. Three different species of potted Chlorophytum Comosum, (Green Chlorophytum Comosum for its green leaves), CC (Combined the leaves of Chlorophytum Comosum with leaves half green and half white) and PC (Purple Chlorophytum Comosum for its purple leaves), were exposed to formaldehyde for 7 days. The results showed formaldehyde removal efficiencies in the daytime were 71.07% ± 0.23, 84.66% ± 0.19, and 46.73% ± 0.15 at 1 ppm for GC, CC, and GC plants, respectively, and were 36.21% ± 0.24, 62.15% ± 0.19, and 34.97% ± 0.11 at night. This might be due to higher plant physiological activities (e.g., photosynthesis, respiration, and transpiration) during the daytime than at night. Ten physiological indicators of leaves were chosen to evaluate the 7-day fumigation process, which were chlorophyll, free protein, relative conductivity, malondialdehyde (MDA), hydrogen peroxide (H2O2), hydroxyl radical, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and total antioxidant capacity (T-AOC). Eight of these indicators increased, while chlorophyll decreased by 22.16%, 6.95%, and 25.32%, and CAT decreased by 18.9%, 17.8%, and 25.30% for GC, CC, and PC respectively. Among all the increasing physiological indicators, relative conductivity and MDA showed the greatest increase by 279.32% and 155.56% for PC. A 15-day recovery study was also conducted using MDA and T-AOC as indicators. The results showed that all the tested plants could be tolerant up to the 8 ppm of formaldehyde concentration for 7 days under dynamic fumigation and needed 10-15 days for self-recovery.

Accumulation of cadmium in potential hyperaccumulators Chlorophytum comosum and Callisia fragrans and role of organic acids under stress conditions.

Cadmium (Cd) accumulation, antioxidant activity (AOA), chlorophyll fluorescence (F) and organic acid distribution in Chlorophytum comosum and Callisia fragrans plants exposed to artificially added Cd (40, 160 and 320 mg kg-1) were examined in pot experiment. At the highest Cd concentration, C. comosum accumulated in roots and the aboveground parts up to 1331 and 1054 mg Cd kg-1 DW, and C. fragrans up to 1427 and 1263 mg Cd kg-1 DW, respectively, which are quite near at the level of hyperaccumulator. Cd accumulation in both plant species increased significantly with the increment of soil Cd dosage, and the distribution was roots > shoots > stolons. Values of BC showed rising trend indicating an accumulation potential of both species. The root AOA was positively correlated to Cd addition, especially in C. comosum. Higher values of free SA were found in roots with a significant enhancement at concentrations of 40 and 160 mg kg-1 Cd. It was observed that citric acid significantly reacted in both species, while fumaric acid only in C. comosum in response to Cd which may contribute to Cd chelation. Our data indicate that both species are suitable for phytoextraction of Cd from contaminated soils which increases their value as ornamentals.

Chlorophytum comosum-bacteria interactions for airborne benzene remediation: Effect of native endophytic Enterobacter sp. EN2 inoculation and blue-red LED light.

This study was performed to determine the effect of plant-endophytic Enterobacter sp. EN2 interactions and blue-red LED light conditions on gaseous benzene removal by plants. It was found that under consecutive benzene fumigation for three cycles (18 days), inoculation of the strain EN2 into sterilized and non-sterilized native C. comosum resulted in significantly increased gaseous benzene removal compared to that in non-inoculated groups under the same light conditions (P < 0.05). Remarkably, EN2 colonization in inoculated plants under LED conditions was higher than under fluorescence conditions as the EN2 could grow better under LED conditions. Strain EN2 possesses NADPH that is used to facilitate benzene degradation and modulate plant growth under benzene stress by bacterial IAA production and ACC deaminase activity; higher IAA and lower ethylene levels were found in inoculated plants compared to non-inoculated ones. These contributed to better benzene removal efficiency. Interestingly, under fumigation for 16 cycles (67 days), there was no difference in gaseous benzene removal between inoculated plants and non-inoculated plants under the same light conditions at initial benzene concentrations of 5 ppm. This is probably due to EN2 reaching maximum growth under all treatments. However, C. comosum exhibited better benzene removal under LED conditions than under fluorescence conditions during 16 cycles, possibly due to better photosynthetic performance and plant growth, leading to more NADPH, and eventually enhanced benzene removal efficiency. Hence, the most efficient acceleration of benzene removal was provided by inoculation of strain EN2 onto C. comosum under blue-red LED light conditions.

The influence of different light quality and benzene on gene expression and benzene degradation of Chlorophytum comosum.

Benzene, a carcinogenic compound, has been reported as a major indoor air pollutant. Chlorophytum comosum (C. comosum) was reported to be the highest efficient benzene removal plant among other screened plants. Our previous studies found that plants under light conditions could remove gaseous benzene higher than under dark conditions. Therefore, C. comosum exposure to airborne benzene was studied under different light quality at the same light intensity. C. comosum could remove 500 ppm gaseous benzene with the highest efficiency of 68.77% under Blue:Red = 1:1 LED treatments and the lowest one appeared 57.41% under white fluorescent treatment within 8 days. After benzene was uptaken by C. comosum, benzene was oxidized to be phenol in the plant cells by cytochrome P450 monooxygenase system. Then, phenol was catalyzed to be catechol that was confirmed by the up-regulation of phenol 2-monooxygenase (PMO) gene expression. After that, catechol was changed to cic, cis-muconic acid. Interestingly, cis,cis-muconic acid production was found in the plant tissues higher than phenol and catechol. The result confirmed that NADPH-cytochrome P450 reductase (CPR), cytochrome b5 (cyt b5), phenol 2-monooxygenase (PMO) and cytochrome P450 90B1 (CYP90B1) in plant cells were involved in benzene degradation or detoxification. In addition, phenol, catechol, and cis,cis-muconic acid production were found under the Blue-Red LED light conditions higher than under white fluorescent light conditions due to under LED light conditions gave higher NADPH contents. Hence, C. comosum under the Blue-Red LED light conditions had a high potential to remove benzene in a contaminated site.

Phosphate fertilizer affected rhizospheric soils: speciation of cadmium and phytoremediation by Chlorophytum comosum.

Experiments were conducted to investigate the effect of phosphate fertilization on chemical speciation of cadmium (Cd) in the rhizospheric soil of Chlorophytum comosum, a potential cadmium hyperaccumulator. The results revealed that when 200 mg kg-1 phosphate was applied into the soil, the Cd contents in the exchangeable fraction (EXC), carbonate-binding fraction (CA), and Fe-Mn oxides-binding fraction (Fe-Mn) were the highest, and the Cd content in the residual fraction (RES) was the lowest. Phosphate fertilization could enhance Cd conversion from RES into CA and weak RES, thereby improving the bioavailability of Cd and enhancing Cd enrichment and adsorption by C. comosum. The total Cd content in the soil was reduced by 10.15 mg kg-1 in the planted group, which was significantly different from the control group (p < 0.01). The highest bioaccumulation coefficient (BC) values in root and aboveground parts appeared when the phosphate rates were 276 and 217 mg kg-1, whereas the highest translocation factor (TF) occurred with a phosphate rate of 188 mg kg-1. Phosphate fertilization facilitated phytoremediation of Cd-polluted soil by C. comosum.

Microbiome interplay: plants alter microbial abundance and diversity within the built environment.

The built indoor microbiome has importance for human health. Residents leave their microbial fingerprint but nothing is known about the transfer from plants. Our hypothesis that indoor plants contribute substantially to the microbial abundance and diversity in the built environment was experimentally confirmed as proof of principle by analyzing the microbiome of the spider plant Chlorophytum comosum in relation to their surroundings. The abundance of Archaea, Bacteria, and Eukaryota (fungi) increased on surrounding floor and wall surfaces within 6 months of plant isolation in a cleaned indoor environment, whereas the microbial abundance on plant leaves and indoor air remained stable. We observed a microbiome shift: the bacterial diversity on surfaces increased significantly but fungal diversity decreased. The majority of cells were intact at the time of samplings and thus most probably alive including diverse Archaea as yet unknown phyllosphere inhabitants. LEfSe and network analysis showed that most microbes were dispersed from plant leaves to the surrounding surfaces. This led to an increase of specific taxa including spore-forming fungi with potential allergic potential but also beneficial plant-associated bacteria, e.g., Paenibacillus. This study demonstrates for the first time that plants can alter the microbiome of a built environment, which supports the significance of plants and provides insights into the complex interplay of plants, microbiomes and human beings.

Rotylenchulus reniformis on Greenhouse-grown Foliage Plants: Host Range and Sources of Inoculum.

Two sources of inoculum of reniform nematodes, Rotylenchulus reniformis, were identified for infestation of ornamental foliage plants in commercial greenhouses. These were water from a local canal system and rooted cuttings purchased from other sources. Eight ornamental plant species were identified as good hosts for the reniform nematode, with each species supporting a reniform population density equal to or greater than that supported by 'Rutgers' tomato and a reproduction factor of greater than 1.0. Nine other plant species were identified as poor hosts.